Apoptosis in breast cancer

Its rate in China and other Asian countries is also increasing rapidly [ 1 ]. These observations are in agreement with our in vitro studies showing that treatment of breast cancer cells with curcumin results in a dose- and time-dependent induction of apoptosis. To test the physiological relevance of in vitro curcumin-induced antitumor effects in vivo, the antitumor effects of curcumin were evaluated in MDA-MB xenografts.

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Apoptosis in breast cancer
Apoptosis in breast cancer

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The MTT assay was used to evaluate cell viability, and flow cytometry, acridine orange staining and transmission electron microscopy were used to detect apoptosis for apoptosis in breast cancer cells. The cell viability index was calculated according to the formula: Int J Mol Sci. Open in a separate window. The medium then was changed to test solutions.

Apoptosis in breast cancer
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Apoptosis in breast cancer
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Developing phytoestrogens for breast cancer prevention. As shown in Figure 6the pro-apoptotic protein Bax was induced in curcumin-treated cells, whereas the expression of antiapoptotic protein Bcl-2 was inhibited after treatment with curcumin for 48 h. In addition, the cellular morphological changes and molecular mechanisms underlying curcumin-induced apoptosis in breast cells have not been well documented.

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